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Lispro NT-EIA背景介紹：

Mercodia Lispro NL-ELISA provides a method for the quantitative determination of insulin lispro in plasma or serum samples.

Lispro NT-EIA   Summary and explanation of the test

The Mercodia Lispro NL-ELISA is a ligand binding assay able to measure insulinlispro specifically, without cross-reaction to native insulin, native proinsulin, or anyof the tested insulin analogues. Also, no interference from insulin autoantibodies(IgG antibodies), which can be present in samples from diabetic patients, hasbeen observed1.Mercodia Lispro NL-ELISA is designed to fit the needs of the pharmaceuticalindustry, by meeting the requirements specified in the EMA2/FDA3 guidelines.Seven serum calibrators (incl. Calibrator 0), two anchor points and three controlsare included in the kit.

Lispro NT-EIA Principle of the procedure

Mercodia Lispro NL-ELISA is a solid phase two-site enzyme immunoassay basedon the sandwich technique, in which two monoclonal antibodies are directedagainst separate antigenic determinants on the lispro molecule. Insulin lispro inthe sample reacts with anti-lispro antibodies bound to microtitration wells andperoxidase-conjugated anti-lispro antibodies in the solution. A simple washingstep removes unbound enzyme-labelled antibody. The bound conjugate isdetected by reaction with the chemiluminescent substrate. A chemiluminescenceplate reader is used to read the intensity of light generated.